Microbox
guidelines for sterilization in the autoclave and optimal gas exchange
Sterilization
in the autoclave
(“autoclaving”)
Preferable procedure: to autoclave containers and medium separately.
This way, PP containers perfectly keep their shape and can be used an infinite number of times over and over again:
· pack stapled containers and lids separately in an autoclavable wrapping
· fill containers under LAF with warm sterilised medium
· click to close lid brims all around
· keep containers with medium in a well ventilated room
Preferable procedure: to autoclave containers and medium separately.
This way, PP containers perfectly keep their shape and can be used an infinite number of times over and over again:
· pack stapled containers and lids separately in an autoclavable wrapping
· fill containers under LAF with warm sterilised medium
· click to close lid brims all around
· keep containers with medium in a well ventilated room
Corner
of lid left open:
no deformation.
gas has free passage
no deformation.
gas has free passage
Hermetically
closed lid:
containers garble with pressure changes.
quick pressure increase or reduction
containers garble with pressure changes.
quick pressure increase or reduction
Alternative
procedure:
to autoclave containers with medium, the following procedures avoid that the containers are deformed or burst while autoclaving.
· fill containers with medium
· place the lids on containers,
· press gently along 3/4 of surface · leave one border of lid open
After sterilisation, slowly reduce the pressure in the autoclave back to atmospheric pressure.
Immediately after emptying the autoclave, click to close the lids all around and keep containers with medium in a well ventilated room.
to autoclave containers with medium, the following procedures avoid that the containers are deformed or burst while autoclaving.
· fill containers with medium
· place the lids on containers,
· press gently along 3/4 of surface · leave one border of lid open
After sterilisation, slowly reduce the pressure in the autoclave back to atmospheric pressure.
Immediately after emptying the autoclave, click to close the lids all around and keep containers with medium in a well ventilated room.
Gas
exchange
By means of diffusion throughout a series of depth filters. The gas exchange is optimal in the following conditions:
· keep the filter zones free of labels or any other object
· keep the filters well dry:
· before re-using the lids, clean them by means of a moist cloth (do not soak!)
· leave a border of the lid open during sterilisation (see above)
· dry accidently wet filters in circulating air
· choose the suitable filter type (L, XL, XXL, XXL+) and adjust ventilation ratio in order to obtain
an ideal gas exchange in combination with a minimal dose of deshydratation
By means of diffusion throughout a series of depth filters. The gas exchange is optimal in the following conditions:
· keep the filter zones free of labels or any other object
· keep the filters well dry:
· before re-using the lids, clean them by means of a moist cloth (do not soak!)
· leave a border of the lid open during sterilisation (see above)
· dry accidently wet filters in circulating air
· choose the suitable filter type (L, XL, XXL, XXL+) and adjust ventilation ratio in order to obtain
an ideal gas exchange in combination with a minimal dose of deshydratation
